International publications using AP-SMALDI technology
  1. Bartels B, Svatos A. Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI. Frontiers in Plant Science. 2015;6. DOI: 10.3389/fpls.2015.00471
  2. Kusari S, Sezgin S, Nigutova K, Cellarova E, Spiteller M. Spatial chemo-profiling of hypericin and related phytochemicals in Hypericum species using MALDI-HRMS imaging. Analytical and Bioanalytical Chemistry. 2015;407(16):4779-91. DOI: 10.1007/s00216-015-8682-6
  3. Wang WX, Kusari S, Sezgin S, Lamshoft M, Kusari P, Kayser O, Spiteller M. Hexacyclopeptides secreted by an endophytic fungus Fusarium solani N06 act as crosstalk molecules in Narcissus tazetta. Applied Microbiology and Biotechnology. 2015;99(18):7651-62. DOI: 10.1007/s00253-015-6653-7
  4. Ait-Belkacem R, Dilillo M, Pellegrini D, Yadav A, de Graaf EL, McDonnell LA. In-Source Decay and Pseudo-MS3 of Peptide and Protein Ions Using Liquid AP-MALDI. Journal of the American Society for Mass Spectrometry. 2016;27(12):2075-9. DOI: 10.1007/s13361-016-1511-0
  5. Beck S, Stengel J. Mass spectrometric imaging of flavonoid glycosides and biflavonoids in Ginkgo biloba L. Phytochemistry. 2016;130:201-6. DOI: 10.1016/j.phytochem.2016.05.005
  6. Eckelmann D, Kusari S, Spiteller M. Occurrence and spatial distribution of maytansinoids in Putterlickia pyracantha, an unexplored resource of anticancer compounds. Fitoterapia. 2016;113:175-81. DOI: 10.1016/j.fitote.2016.08.006
  7. Kucharikova A, Kusari S, Sezgin S, Spiteller M, Cellarova E. Occurrence and Distribution of Phytochemicals in the Leaves of 17 In vitro Cultured Hypericum spp. Adapted to Outdoor Conditions. Frontiers in Plant Science. 2016;7. DOI: 10.3389/fpls.2016.01616
  8. Mascini NE, Cheng ML, Jiang L, Rizwan A, Podmore H, Bhandari DR, Rompp A, Glunde K, Heeren RMA. Mass Spectrometry Imaging of the Hypoxia Marker Pimonidazole in a Breast Tumor Model. Analytical Chemistry. 2016;88(6):3107-14. DOI: 10.1021/acs.analchem.5b04032
  9. Belov ME, Ellis SR, Dilillo M, Paine MRL, Danielson WF, Anderson GA, de Graaf EL, Eijkel GB, Heeren RMA, McDonnell LA. Design and Performance of a Novel Interface for Combined Matrix-Assisted Laser Desorption Ionization at Elevated Pressure and Electrospray Ionization with Orbitrap Mass Spectrometry. Analytical Chemistry. 2017;89(14):7493-501. DOI: 10.1021/acs.analchem.7b01168
  10. Breindahl T, Kimergard A, Andreasen MF, Pedersen DS. Identification of a new psychoactive substance in seized material: the synthetic opioid N-phenyl-N- 1-(2-phenethyl)piperidin-4-yl prop-2-enamide (Acrylfentanyl). Drug Testing and Analysis. 2017;9(3):415-22. DOI: 10.1002/dta.2046
  11. Dilillo M, Pellegrini D, Ait-Belkacem R, de Graaf EL, Caleo M, McDonnell LA. Mass Spectrometry Imaging, Laser Capture Microdissection, and LC-MS/MS of the Same Tissue Section. Journal of Proteome Research. 2017;16(8):2993-3001. DOI: 10.1021/acs.jproteome.7b00284
  12. Eckelmann D, Kusari S, Spiteller M. Spatial profiling of maytansine during the germination process of Maytenus senegalensis seeds. Fitoterapia. 2017;119:51-6. DOI: 10.1016/j.fitote.2017.03.014
  13. Nishidate M, Yamamoto K, Masuda C, Aikawa H, Hayashi M, Kawanishi T, Hamada A. MALDI mass spectrometry imaging of erlotinib administered in combination with bevacizumab in xenograft mice bearing B901L, EGFR-mutated NSCLC cells. Scientific Reports. 2017;7. DOI: 10.1038/s41598-017-17211-6
  14. Sorensen IS, Janfelt C, Nielsen MMB, Mortensen RW, Knudsen NO, Eriksson AH, Pedersen AJ, Nielsen KT. Combination of MALDI-MSI and cassette dosing for evaluation of drug distribution in human skin explant. Analytical and Bioanalytical Chemistry. 2017;409(21):4993-5005. DOI: 10.1007/s00216-017-0443-2
  15. Wenande E, Olesen UH, Nielsen MMB, Janfelt C, Hansen SH, Anderson RR, Haedersdal M. Fractional laser-assisted topical delivery leads to enhanced, accelerated and deeper cutaneous 5-fluorouracil uptake. Expert Opin Drug Deliv. 2017;14(3):307-17. DOI: 10.1080/17425247.2017.1260119
  16. Eckelmann D, Spiteller M, Kusari S. Spatial-temporal profiling of prodiginines and serratamolides produced by endophytic Serratia marcescens harbored in Maytenus serrata. Scientific Reports. 2018;8. DOI: 10.1038/s41598-018-23538-5
  17. Ha-Andersen J, Kaasgaard SG, Janfelt C. MALDI imaging of enzymatic degradation of glycerides by lipase on textile surface. Chemistry and Physics of Lipids. 2018;211:100-6. DOI: 10.1016/j.chemphyslip.2017.11.004
  18. Hansen SE, Marxen E, Janfelt C, Jacobsen J. Buccal delivery of small molecules - Impact of levulinic acid, oleic acid, sodium dodecyl sulfate and hypotonicity on ex vivo permeability and spatial distribution in mucosa. European Journal of Pharmaceutics and Biopharmaceutics. 2018;133:250-7. DOI: 10.1016/j.ejpb.2018.10.016
  19. Kashimura A, Tanaka K, Sato H, Kaji H, Tanaka M. Imaging mass spectrometry for toxicity assessment: a useful technique to confirm drug distribution in histologically confirmed lesions. Journal of Toxicologic Pathology. 2018;31(3):221-7. DOI: 10.1293/tox.2018-0006
  20. Kohnen KL, Sezgin S, Spiteller M, Hagels H, Kayser O. Localization and Organization of Scopolamine Biosynthesis in Duboisia myoporoides R. Br. Plant and Cell Physiology. 2018;59(1):107-18. DOI: 10.1093/pcp/pcx165
  21. Marxen E, Jacobsen J, Hyrup B, Janfelt C. Permeability Barriers for Nicotine and Mannitol in Porcine Buccal. Mucosa Studied by High-Resolution MALDI Mass Spectrometry Imaging. Molecular Pharmaceutics. 2018;15(2):519-26. DOI: 10.1021/acs.molpharmaceut.7b00891
  22. Marxen E, Jin L, Jacobsen J, Janfelt C, Hyrup B, Nicolazzo JA. Effect of Permeation Enhancers on the Buccal Permeability of Nicotine: Ex vivo Transport Studies Complemented by MALDI MS Imaging. Pharmaceutical Research. 2018;35(3). DOI: 10.1007/s11095-017-2332-y
  23. Tocci N, Gaid M, Kaftan F, Belkheir AK, Belhadj I, Liu BY, Svatos A, Hansch R, Pasqua G, Beerhues L. Exodermis and endodermis are the sites of xanthone biosynthesis in Hypericum perforatum roots. New Phytologist. 2018;217(3):1099-112. DOI: 10.1111/nph.14929
  24. Yakoub K, Jung SS, Sattler C, Damerow H, Weber J, Kretzschmann A, Cankaya AS, Piel M, Rosch F, Haugaard AS, Frolund B, Schirmeister T, Luddens H. Structure Function Evaluation of Imidazopyridine Derivatives Selective for delta-Subunit-Containing gamma-Aminobutyric Acid Type A (GABA(A)) Receptors. Journal of Medicinal Chemistry. 2018;61(5):1951-68. DOI: 10.1021/acs.jmedchem.7b01484
  25. Baumeister TUH, Vallet M, Kaftan F, Svatos A, Pohnert G. Live Single-Cell Metabolomics With Matrix-Free Laser/Desorption Ionization Mass Spectrometry to Address Microalgal Physiology. Frontiers in Plant Science. 2019;10. DOI: 10.3389/fpls.2019.00172
  26. Clitherow KH, Murdoch C, Spain SG, Handler AM, Colley HE, Stie MB, Nielsen HM, Janfelt C, Hatton PV, Jacobsen J. Mucoadhesive Electrospun Patch Delivery of Lidocaine to the Oral Mucosa and Investigation of Spatial Distribution in a Tissue Using MALDI-Mass Spectrometry Imaging. Molecular Pharmaceutics. 2019;16(9):3948-56. DOI: 10.1021/acs.molpharmaceut.9b00535
  27. Handler AM, Marxen E, Jacobsen J, Janfelt C. Visualization of the penetration modifying mechanism of laurocapram by Mass Spectrometry Imaging in buccal drug delivery. European Journal of Pharmaceutical Sciences. 2019;127:276-81. DOI: 10.1016/j.ejps.2018.11.011
  28. Hendel KK, Bagger C, Olesen UH, Janfelt C, Hansen SH, Haedersdal M, Lerche CM. Fractional laser-assisted topical delivery of bleomycin quantified by LC-MS and visualized by MALDI mass spectrometry imaging. Drug Delivery. 2019;26(1):244-51. DOI: 10.1080/10717544.2019.1574937
  29. Kubicki M, Lamshoft M, Lagojda A, Spiteller M. Metabolism and spatial distribution of metalaxyl in tomato plants grown under hydroponic conditions. Chemosphere. 2019;218:36-41. DOI: 10.1016/j.chemosphere.2018.11.069
  30. Nishidate M, Hayashi M, Aikawa H, Tanaka K, Nakada N, Miura S, Ryu S, Higashi T, Ikarashi Y, Fujiwara Y, Hamada A. Applications of MALDI mass spectrometry imaging for pharmacokinetic studies during drug development. Drug Metab Pharmacokinet. 2019;34(4):209-16. DOI: 10.1016/j.dmpk.2019.04.006
  31. Sugiyama E, Kondo T, Kuzumaki N, Honda K, Yamanaka A, Narita M, Suematsu M, Sugiura Y. Mechanical allodynia induced by optogenetic sensory nerve excitation activates dopamine signaling and metabolism in medial nucleus accumbens. Neurochem Int. 2019;129:6. DOI: 10.1016/j.neuint.2019.104494
  32. Takahashi M, Miki S, Fujimoto K, Fukuoka K, Matsushita Y, Maida Y, Yasukawa M, Hayashi M, Shinkyo R, Kikuchi K, Mukasa A, Nishikawa R, Tamura K, Narita Y, Hamada A, Masutomi K, Ichimura K. Eribulin penetrates brain tumor tissue and prolongs survival of mice harboring intracerebral glioblastoma xenografts. Cancer Science. 2019;110(7):2247-57. DOI: 10.1111/cas.14067
  33. Baumeister TUH, Vallet M, Kaftan F, Guillou L, Svatos A, Pohnert G. Identification to species level of live single microalgal cells from plankton samples with matrix-free laser/desorption ionization mass spectrometry. Metabolomics. 2020;16(3):10. DOI: 10.1007/s11306-020-1646-7
  34. Kalouta K, Stie MB, Janfelt C, Chronakis IS, Jacobsen J, Nielsen HM, et al. Electrospun alpha-Lactalbumin Nanofibers for Site-Specific and Fast-Onset Delivery of Nicotine in the Oral Cavity: An In Vitro, Ex Vivo, and Tissue Spatial Distribution Study. Molecular Pharmaceutics. 2020;17(11):4189-200. DOI: 10.1021/acs.molpharmaceut.0c00642
  35. Kyekyeku JO, Asare-Nkansah S, Bekoe SO, Sezgin S, Adosraku RK, Spiteller M. MALDI-HRMS imaging and HPLC-HRESI-MSn characterisation of kaurane diterpenes in the fruits of Xylopia aethiopica (Dunal) A. Rich (Annonaceae). Phytochemical Analysis. 2020;31(3):349-54. DOI: 10.1002/pca.2901
  36. Tanaka M, Saka-Tanaka M, Ochi K, Fujieda K, Sugiura Y, Miyamoto T, et al. C-type lectin Mincle mediates cell death-triggered inflammation in acute kidney injury. Journal of Experimental Medicine. 2020;217(11):21. DOI: 10.1084/jem.20192230
  37. Treu A, Kokesch-Himmelreich J, Walter K, Holscher C, Rompp A. Integrating High-Resolution MALDI Imaging into the Development Pipeline of Anti-Tuberculosis Drugs. Journal of the American Society for Mass Spectrometry. 2020;31(11):2277-86. DOI: 10.1021/jasms.0c00235
  38. Trombetta D, Smeriglio A, Denaro M, Zagami R, Tomassetti M, Pilolli R, et al. Understanding the Fate of Almond (Prunus dulcis (Mill.) DA Webb) Oleosomes during Simulated Digestion. Nutrients. 2020;12(11):17. DOI: 10.3390/nu12113397
  39. Tsugawa H, Kabe Y, Kanai A, Sugiura Y, Hida S, Taniguchi S, et al. Short-chain fatty acids bind to apoptosis-associated speck-like protein to activate inflammasome complex to preventSalmonellainfection. Plos Biology. 2020;18(9):34. DOI: 10.1371/journal.pbio.3000813
  40. Handler AM, Pedersen GP, Nielsen KT, Janfelt C, Pedersen AJ, Clench MR. Quantitative MALDI mass spectrometry imaging for exploring cutaneous drug delivery of tofacitinib in human skin. European Journal of Pharmaceutics and Biopharmaceutics. 2021;159:1-10. DOI: 10.1016/j.ejpb.2020.12.008
  41. Iwama T, Kano K, Saigusa D, Ekroos K, van Echten-Deckert G, Vogt J, et al. Development of an On-Tissue Derivatization Method for MALDI Mass Spectrometry Imaging of Bioactive Lipids Containing Phosphate Monoester Using Phos-tag. Analytical Chemistry. 2021;93(8):3867-75. DOI: 10.1021/acs.analchem.0c04479
  42. Rosenberg LK, Bagger C, Janfelt C, Haedersdal M, Olesen UH, Lerche CM. A Comparison of Human and Porcine Skin in Laser-Assisted Drug Delivery of Chemotherapeutics. Lasers in Surgery and Medicine. 2021;53(1):162-70. DOI: 10.1002/lsm.23344
  43. Wenande E, Hendel K, Mogensen M, Bagger C, Martensson NL, Persson DP, et al. Efficacy and Safety of Laser-Assisted Combination Chemotherapy: An Explorative Imaging-Guided Treatment With 5-Fluorouracil and Cisplatin for Basal Cell Carcinoma. Lasers in Surgery and Medicine. 2021;53(1):119-28. DOI: 10.1002/lsm.23323
  44. Handler, A. M.; Eirefelt, S.; Lambert, M.; Johansson, F.; Schefe, L. H.; Knudsen, N. O.; Bodenlenz, M.; Birngruber, T.; Sinner, F.; Eriksson, A. H.; et al. Characterizing Cutaneous Drug Delivery Using Open-Flow Microperfusion and Mass Spectrometry Imaging. Molecular Pharmaceutics 2021, 18 (8), 3063-3072. DOI: 10.1021/acs.molpharmaceut.1c00285.
  45. Strindberg, S.; Plum, J.; Bagger, C.; Janfelt, C.; Mullertz, A. Visualizing the Journey of Fenofibrate through the Rat Gastrointestinal Tract by Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging. Molecular Pharmaceutics 2021, 18 (6), 2189-2197. DOI: 10.1021/acs.molpharmaceut.0c01043.
  46. Hendel, K.; Hansen, A. C. N.; Bik, L.; Bagger, C.; Van Doorn, M. B. A.; Janfelt, C.; Olesen, U. H.; Haedersdal, M.; Lerche, C. M. Bleomycin administered by laser-assisted drug delivery or intradermal needle-injection results in distinct biodistribution patterns in skin: in vivo investigations with mass spectrometry imaging. Drug Delivery 2021, 28 (1), 1141-1149. DOI: 10.1080/10717544.2021.1933649.
  47. Bik, L.; van Doorn, M.; Hansen, A. C. N.; Janfelt, C.; Olesen, U. H.; Haedersdal, M.; Lerche, C. M.; Hendel, K. In vivo dermal delivery of bleomycin with electronic pneumatic injection: drug visualization and quantification with mass spectrometry. Expert Opinion on Drug Delivery. DOI: 10.1080/17425247.2022.2035719.
  48. Granborg, J. R.; Kaasgaard, S. G.; Janfelt, C. Mass spectrometry imaging of oligosaccharides following in situ enzymatic treatment of maize kernels. Carbohydrate Polymers 2022, 275. DOI: 10.1016/j.carbpol.2021.118693.
  49. Holm, N. B.; Deryabina, M.; Knudsen, C. B.; Janfelt, C. Tissue distribution and metabolic profiling of cyclosporine (CsA) in mouse and rat investigated by DESI and MALDI mass spectrometry imaging (MSI) of whole-body and single organ cryo-sections. Analytical and Bioanalytical Chemistry. DOI: 10.1007/s00216-022-04269-z.
  50. Patric Bourceau, D. M., Benedikt Geier, Manuel Liebeke​. Spatial metabolomics shows contrasting phosphonolipid distributions in tissues of marine bivalves. PeerJ Analytical Chemistry 2022. DOI: 10.7717/peerj-achem.21.
  51. Du MY, Yin ZB, Xu KJ, Huang YD, Xu YZ, Wen WL, et al. Integrated mass spectrometry imaging and metabolomics reveals sublethal effects of indoxacarb on the red fire ant Solenopsis invicta. Pest Management Science. 2023. DOI: 10.1002/ps.7489.
  52. Hao C, Yang W, Dong G, Yu Y, Liu Y, Zhang J, et al. Visualization and identification of benzylisoquinoline alkaloids in various. Heliyon. 2023;9(6):e16138. DOI: 10.1016/j.heliyon.2023.e16138.
  53. Granborg JR, Kaasgaard SG, Janfelt C. Variation in oligosaccharide profiles observed with AP-MALDI in different regions of maize kernels after treatment with xylanases. Journal of Cereal Science. 2023;109. DOI: 10.1016/j.jcs.2022.103586.
  54. Bidesi N, Shalgunov V, Battisti UM, Hvass L, Jorgensen JT, Poulie CBM, et al. Synthesis and radiolabeling of a polar I-125 I-1,2,4,5-tetrazine. Journal of Labelled Compounds & Radiopharmaceuticals. 2023;66(1):22-30. DOI: 10.1002/jlcr.4009.
  55. Vallet M, Kaftan F, Buaya A, Thines M, Guillou L, Svatoš A, et al. Single-cell metabolome profiling for phenotyping parasitic diseases in phytoplankton. Frontiers in Analytical Science. 2023;2. DOI: 10.3389/frans.2022.1051955.
  56. Badiali C, Petruccelli V, Brasili E, Pasqua G. Xanthones: Biosynthesis and Trafficking in Plants, Fungi and Lichens. Plants-Basel. 2023;12(4). DOI: 10.3390/plants12040694.
  57. Chen JY, Mao LC, Jiang YM, Liu HH, Wang X, Meng LW, et al. Revealing the In Situ Behavior of Aggregation-Induced Emission Nanoparticles and Their Biometabolic Effects via Mass Spectrometry Imaging. Acs Nano. 2023;17(5):4463-73. DOI: 10.1021/acsnano.2c10058
Publications in collaboration with the working group of Prof. Spengler
  1. Rompp A, Guenther S, Schober Y, Schulz O, Takats Z, Kummer W, Spengler B. Histology by Mass Spectrometry: Label-Free Tissue Characterization Obtained from High-Accuracy Bioanalytical Imaging. Angewandte Chemie-International Edition. 2010;49(22):3834-8. DOI: 10.1002/anie.200905559
  2. Guenther S, Rompp A, Kummer W, Spengler B. AP-MALDI imaging of neuropeptides in mouse pituitary gland with 5 mu m spatial resolution and high mass accuracy. International Journal of Mass Spectrometry. 2011;305(2-3):228-37. DOI: 10.1016/j.ijms.2010.11.011
  3. Rompp A, Guenther S, Takats Z, Spengler B. Mass spectrometry imaging with high resolution in mass and space (HR2 MSI) for reliable investigation of drug compound distributions on the cellular level. Analytical and Bioanalytical Chemistry. 2011;401(1):65-73. DOI: 10.1007/s00216-011-4990-7
  4. Schober Y, Guenther S, Spengler B, Rompp A. Single Cell Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging. Analytical Chemistry. 2012;84(15):6293-7. DOI: 10.1021/ac301337h
  5. Schober Y, Guenther S, Spengler B, Rompp A. High-resolution matrix-assisted laser desorption/ionization imaging of tryptic peptides from tissue. Rapid Communications in Mass Spectrometry. 2012;26(9):1141-6. DOI: 10.1002/rcm.6192
  6. Paschke C, Leisner A, Hester A, Maass K, Guenther S, Bouschen W, Spengler B. Mirion-A Software Package for Automatic Processing of Mass Spectrometric Images. Journal of the American Society for Mass Spectrometry. 2013;24(8):1296-306. DOI: 10.1007/s13361-013-0667-0
  7. Rompp A, Spengler B. Mass spectrometry imaging with high resolution in mass and space. Histochemistry and Cell Biology. 2013;139(6):759-83. DOI: 10.1007/s00418-013-1097-6
  8. Berisha A, Dold S, Guenther S, Desbenoit N, Takats Z, Spengler B, Rompp A. A comprehensive high-resolution mass spectrometry approach for characterization of metabolites by combination of ambient ionization, chromatography and imaging methods. Rapid Communications in Mass Spectrometry. 2014;28(16):1779-91. DOI: 10.1002/rcm.6960
  9. Bhandari DR, Shen T, Rompp A, Zorn H, Spengler B. Analysis of cyathane-type diterpenoids from Cyathus striatus and Hericium erinaceus by high-resolution MALDI MS imaging. Analytical and Bioanalytical Chemistry. 2014;406(3):695-704. DOI: 10.1007/s00216-013-7496-7
  10. Li B, Bhandari DR, Janfelt C, Rompp A, Spengler B. Natural products in Glycyrrhiza glabra (licorice) rhizome imaged at the cellular level by atmospheric pressure matrix-assisted laser desorption/ionization tandem mass spectrometry imaging. Plant Journal. 2014;80(1):161-71. DOI: 10.1111/tpj.12608
  11. Bhandari DR, Schott M, Rompp A, Vilcinskas A, Spengler B. Metabolite localization by atmospheric pressure high-resolution scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging in whole-body sections and individual organs of the rove beetle Paederus riparius. Analytical and Bioanalytical Chemistry. 2015;407(8):2189-201. DOI: 10.1007/s00216-014-8327-1
  12. Bhandari DR, Wang Q, Friedt W, Spengler B, Gottwald S, Rompp A. High resolution mass spectrometry imaging of plant tissues: towards a plant metabolite atlas. Analyst. 2015;140(22):7696-709. DOI: 10.1039/c5an01065a
  13. Khalil SM, Rompp A, Pretzel J, Becker K, Spengler B. Phospholipid Topography of Whole-Body Sections of the Anopheles stephensi Mosquito, Characterized by High-Resolution Atmospheric-Pressure Scanning Microprobe Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging. Analytical Chemistry. 2015;87(22):11309-16. DOI: 10.1021/acs.analchem.5602781
  14. Li B, Bhandari DR, Rompp A, Spengler B. High-resolution MALDI mass spectrometry imaging of gallotannins and monoterpene glucosides in the root of Paeonia lactiflora. Scientific Reports. 2016;6. DOI: 10.1038/srep36074
  15. Nielsen MMB, Lambertsen KL, Clausen BH, Meyer M, Bhandari DR, Larsen ST, Poulsen SS, Spengler B, Janfelt C, Hansen HS. Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia. Scientific Reports. 2016;6. DOI: 10.1038/srep39571
  16. Tsai YH, Bhandari DR, Garrett TJ, Carter CS, Spengler B, Yost RA. Skeletal muscle fiber analysis by atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometric imaging at high mass and high spatial resolution. Proteomics. 2016;16(11-12):1822-4. DOI: 10.1002/pmic.201500536
  17. Khalil SM, Pretzel J, Becker K, Spengler B. High-resolution AP-SA/LUDT mass spectrometry imaging of Drosophila melanogaster. International Journal of Mass Spectrometry. 2017;416:1-19. DOI: 10.1016/j.ijms.2017.04.001
  18. Kompauer M, Heiles S, Spengler B. Autofocusing MALDI mass spectrometry imaging of tissue sections and 3D chemical topography of nonflat surfaces. Nature Methods. 2017;14(12):1156-+. DOI: 10.1038/nmeth.4433
  19. Kompauer M, Heiles S, Spengler B. Atmospheric pressure MALDI mass spectrometry imaging of tissues and cells at 1.4-mu m lateral resolution. Nature Methods. 2017;14(1):90-6. DOI: 10.1038/nmeth.4071
  20. Vijayan V, Srinu T, Karnati S, Garikapati V, Linke M, Kamalyan L, Mali SR, Sudan K, Kollas A, Schmid T, Schulz S, Spengler B, Weichhart T, Immenschuh S, Baumgart-Vogt E. A New Immunomodulatory Role for Peroxisomes in Macrophages Activated by the TLR4 Ligand Lipopolysaccharide. Journal of Immunology. 2017;198(6):2414-25. DOI: 10.4049/jimmunol.1601596
  21. Bhandari DR, Wang Q, Li B, Friedt W, Rompp A, Spengler B, Gottwald S. Histology-guided high-resolution AP-SMALDI mass spectrometry imaging of wheat-Fusarium graminearum interaction at the root-shoot junction. Plant Methods. 2018;14. DOI: 10.1186/s13007-018-0368-6
  22. Desbenoit N, Walch A, Spengler B, Brunelle A, Rompp A. Correlative mass spectrometry imaging, applying time-of-flight secondary ion mass spectrometry and atmospheric pressure matrix-assisted laser desorption/ionization to a single tissue section. Rapid Communications in Mass Spectrometry. 2018;32(2):159-66. DOI: 10.1002/rcm.8022
  23. Huber K, Khamehgir-Silz P, Schramm T, Gorshkov V, Spengler B, Rompp A. Approaching cellular resolution and reliable identification in mass spectrometry imaging of tryptic peptides. Analytical and Bioanalytical Chemistry. 2018;410(23):5825-37. DOI: 10.1007/s00216-018-1199-z
  24. Khamehgir-Silz P, Schnitter F, Wagner AH, Gerbig S, Schulz S, Hecker M, Spengler B. Strategy for marker-based differentiation of pro- and anti-inflammatory macrophages using matrix-assisted laser desorption/ionization mass spectrometry imaging. Analyst. 2018;143(18):4273-82. DOI: 10.1039/c8an00659h
  25. Pleik S, Spengler B, Bhandari DR, Luhn S, Schafer T, Urbach D, Kirsch D. Ambient-air ozonolysis of triglycerides in aged fingerprint residues. Analyst. 2018;143(5):1197-209. DOI: 10.1039/c7an01506b
  26. Schaepe K, Bhandari DR, Werner J, Henss A, Pirkl A, Kleine-Boymann M, Rohnke M, Wenisch S, Neumann E, Janek J, Spengler B. Imaging of Lipids in Native Human Bone Sections Using TOF-Secondary Ion Mass Spectrometry, Atmospheric Pressure Scanning Microprobe Matrix-Assisted Laser Desorption/Ionization Orbitrap-Mass Spectrometry, and Orbitrap-Secondary Ion Mass Spectrometry. Analytical Chemistry. 2018;90(15):8856-64. DOI: 10.1021/acs.analchem.8b00892
  27. Bader S, Gerbig S, Spengler B, Schwiertz A, Breves G, Diener M. Robustness of the non-neuronal cholinergic system in rat large intestine against luminal challenges. Pflugers Archiv-European Journal of Physiology. 2019;471(4):605-18. DOI: 10.1007/s00424-018-2236-7
  28. Garikapati V, Karnati S, Bhandari DR, Baumgart-Vogt E, Spengler B. High-resolution atmospheric-pressure MALDI mass spectrometry imaging workflow for lipidomic analysis of late fetal mouse lungs. Scientific Reports. 2019;9. DOI: 10.1038/s41598-019-39452-3
  29. Kadesch P, Quack T, Gerbig S, Grevelding CG, Spengler B. Lipid Topography in Schistosoma mansoni Cryosections, Revealed by Microembedding and High-Resolution Atmospheric-Pressure Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry Imaging. Analytical Chemistry. 2019;91(7):4520-8. DOI: 10.1021/acs.analchem.8b05440
  30. Geier B, Sogin EM, Michellod D, Janda M, Kompauer M, Spengler B, et al. Spatial metabolomics of in situ host-microbe interactions at the micrometre scale. Nature Microbiology. 2020;5(3):498-+. DOI: 10.1038/s41564-019-0664-6
  31. Heiles S, Kompauer M, Muller MA, Spengler B. Atmospheric-Pressure MALDI Mass Spectrometry Imaging at 213 nm Laser Wavelength. Journal of the American Society for Mass Spectrometry. 2020;31(2):326-35. DOI: 10.1021/jasms.9b00052
  32. Kadesch P, Hollubarsch T, Gerbig S, Schneider L, Silva LMR, Hermosilla C, et al. Intracellular Parasites Toxoplasma gondii and Besnoitia besnoiti, Unveiled in Single Host Cells Using AP-SMALDI MS Imaging. Journal of the American Society for Mass Spectrometry. 2020;31(9):1815-24. DOI: 10.1021/jasms.0c00043
  33. Kadesch P, Quack T, Gerbig S, Grevelding CG, Spengler B. Tissue- and sex-specific lipidomic analysis of Schistosoma mansoni using high-resolution atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging. Plos Neglected Tropical Diseases. 2020;14(5):17. DOI: 10.1371/journal.pntd.0008145
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